وليد خالد احمد نجم
  • screening for Intercellular Adhesion Gene Cluster Gene Staphylococcus Isolated form UTI Cases
  • Abstract:

    144 urine samples were collected from persons suspected of urinary
    tract infections, their ages ranged from 7 to 60 years, Some pathogens of urinary
    tract infections were investigated, diagnosed, and antibiotic sensitivity tested. The
    percentage of patients with diabetes and pregnant women was also investigated, in
    addition to comparing patients between age and gender. The results showed the
    results of isolation and diagnosis of Staphylococeus aureus showed 15.27% of
    the number of (22) samples and were divided into age groups. The present study
    evaluated the ability of biofilm formation and the presence of (icaA) and (icaD)
    genes among strains of staphylococci isolated from patients with urinary tract
    infection. The presence of (icaA) and (icaD) gene was also determined by PCR
    technique the Biofilm development is common among Staphylococcus aureus
    isolated from urinary tract infections The sensitivity of Staphylococcus aureus
    bacteria was tested to 10 antibiotics, and the highest sensitivity was to an
    antibiotic Methicillin 91%, and 86.3% towards Amoxicillin. It also gave an
    81% percentage to an antibiotic Levofloxacin and Gentamicin. It is also being less
    sensitive to an antibiotic Ethromycin. The bacteria showed moderate sensitivity to
    an antibiotic Rifampicin 86.36% and to an antibiotic vancomycin 81.81% and it
    was less moderate sensitivity to an antibiotie Methicillin and Ethromycin 4.54%.
    The bacteria showed a high resistance to an antibiotic Ethromycin 90.90% and
    also to an antibiotic Tetracycline 81.81%, While there was no resistance to an
    antibiotic Rifampicin 0.0%. Through the study of the statistical analysis, it was
    shown that there are significant differences, that is, that there are differences in
    the effect of antibiotics on bacteria, and it was the best four antibiotics and had an
    effect on the sensitivity of bacteria it is Methicillin, Gentamicin, Levofloxacin and
    Ciprofloxacin.