screening for Intercellular Adhesion Gene Cluster Gene Staphylococcus Isolated form UTI Cases

Abstract:

144 urine samples were collected from persons suspected of urinary
tract infections, their ages ranged from 7 to 60 years, Some pathogens of urinary
tract infections were investigated, diagnosed, and antibiotic sensitivity tested. The
percentage of patients with diabetes and pregnant women was also investigated, in
addition to comparing patients between age and gender. The results showed the
results of isolation and diagnosis of Staphylococeus aureus showed 15.27% of
the number of (22) samples and were divided into age groups. The present study
evaluated the ability of biofilm formation and the presence of (icaA) and (icaD)
genes among strains of staphylococci isolated from patients with urinary tract
infection. The presence of (icaA) and (icaD) gene was also determined by PCR
technique the Biofilm development is common among Staphylococcus aureus
isolated from urinary tract infections The sensitivity of Staphylococcus aureus
bacteria was tested to 10 antibiotics, and the highest sensitivity was to an
antibiotic Methicillin 91%, and 86.3% towards Amoxicillin. It also gave an
81% percentage to an antibiotic Levofloxacin and Gentamicin. It is also being less
sensitive to an antibiotic Ethromycin. The bacteria showed moderate sensitivity to
an antibiotic Rifampicin 86.36% and to an antibiotic vancomycin 81.81% and it
was less moderate sensitivity to an antibiotie Methicillin and Ethromycin 4.54%.
The bacteria showed a high resistance to an antibiotic Ethromycin 90.90% and
also to an antibiotic Tetracycline 81.81%, While there was no resistance to an
antibiotic Rifampicin 0.0%. Through the study of the statistical analysis, it was
shown that there are significant differences, that is, that there are differences in
the effect of antibiotics on bacteria, and it was the best four antibiotics and had an
effect on the sensitivity of bacteria it is Methicillin, Gentamicin, Levofloxacin and
Ciprofloxacin.